Analysis of the elemental concentrations in rat femur's head using energy dispersive X ray fluorescence spectrometry

EDXRF spectrometry is used normally to determine the concentration of the different elements in a sample with advantages of good sensitivity, multielemental capabilities, fast, non-destructive and because of this simple relation to the fundamental physics of atom-radiation interaction, make of EDXRF a highly attractive analytical technique. A major goal for research in biomedical sciences over the last few decades has been determining the concentrations of various elements in human tissues. In recent years, large increase in research work related to bio-medical field such as blood, human hair, human teeth and bones have mad it desirable to improve the application of EDXRF analysis. The aim of this research is determine the concentration of the elements in femur heads of female and males Wistar rats. For such, samples of rats’ femur of 92 days of age were irradiated with a miniaturized and lowpower X-ray generators (26.3 W and molybdenum anode). In this preliminary study, the results shows that EDXRF can be used to determinate the mains elements presents in femur's head and encourage the authors to investigate others regions of those samples to correlate and know about some pathologic such as osteoporosis or similar disease.